Low-autofluorescence, transparent composite for multiphoton 3D printing

Multiphoton lithography allows the high resolution, free-form 3D printing of structures such as micro-optical elements and scaffolds for Tissue Engineering. A major obstacle in its application these fields is material structure autofluorescence. Existing photoresists promise near zero fluorescence at expense poor mechanical properties, low efficiency. Sudan Black B a molecular quencher used dye biological studies means decreasing autofluorescence polymers. In our study, we report use both photoinitiator post-fabrication treatment step, using zirconium silicate SZ2080 development non-fluorescent composite. We this elements, meso-scale mesenchymal stem cell cultures. Our results show that photosensitive hybrid can be fabrication highly transparent, autofluorescence-free microstructures.